Tunable Wide-Field Illumination and Single-Molecule Photoswitching with a Single MEMS Mirror
نویسندگان
چکیده
Homogeneous illumination in single-molecule localization microscopy (SMLM) is key for the quantitative analysis of super-resolution images. Therefore, different approaches flat-field have been introduced as alternative to conventional Gaussian illumination. Here, we introduce a single microelectromechanical systems (MEMS) mirror tunable and cost-effective device adapting wide-field SMLM. In mode MEMS allowed consistent SMLM metrics across entire field view. Employing photoswitching, developed simple yet powerful routine benchmark schemes on basis local emitter brightness ON-state lifetime. Moreover, propose that tuning beyond optimal conditions enables study kinetics photoswitchable fluorophores within acquisition.
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ژورنال
عنوان ژورنال: ACS Photonics
سال: 2021
ISSN: ['2330-4022']
DOI: https://doi.org/10.1021/acsphotonics.1c00843